The Univeristy of Melbourne The Royal Melbourne Hopspital

A joint venture between The University of Melbourne and The Royal Melbourne Hospital

EDUCATION

Research Projects

Project: Monitoring O-linked glycosylation dynamics within Burkholderia cenocepacia

Scott group

The goal of this project is to understand how the occupancy of glycosylation sites change in response to different growth conditions and perturbations of the key glycosylation enzyme pglL. Using a combination of proteomics and gene silencing with CRISPRi we aim to understand which glycoproteins are preferentially glycosylated to dissect the protein features which govern glycosylation. By better understanding the mechanisms which influence the rate at which a protein becomes glycosylated this may one day improve the efficiency of generating novel glycoconjugates, such as vaccines, using bacterial glycosylation systems.

Contact project supervisor for further
information and application enquiries

Project Supervisor

Dr Nichollas Scott

Project availability
PhD/MPhil
Master of Biomedical Science
Honours

Scott group

nichollas.scott@unimelb.edu.au

2 vacancies

Themes
Antimicrobial Resistance and Healthcare Associated Infections
Host Pathogens Interactions
Cross Cutting Disciplines
Discovery Research

The Scott Group focuses on the application of molecular microbiology and mass spectrometry (MS)-based methodologies to characterise microbial systems. The key focus of the lab is understanding how pathogens of the Burkholderia genus cause disease and why proteins decorated with carbohydrates influence Burkholderia pathogenesis.

Projects within the Scott lab: Two broad projects are currently on offer in the Scott lab; Project 1 involves using proteomics and CRISPRi based approaches to understand which glycoproteins are preferentially glycosylated within Burkholderia cenocepacia. Project 2 involves using proteomics to study the impact of Burkholderia cenocepacia infections on Eukaryotic cells using a combination of proteomics approaches.


Scott group Current Projects