The Univeristy of Melbourne The Royal Melbourne Hopspital

A joint venture between The University of Melbourne and The Royal Melbourne Hospital

EDUCATION

Research Projects

Project: Immunoregulatory functions of the MARCH family of ubiquitin ligases

Villadangos Group

Protein localisation and abundance (proteostasis) are controlled in eukaryotic cells by regulatory pathways, which remain poorly understood. These pathways regulate changes in protein expression or localisation, in response to environmental cues such as the presence of pathogens. Addition of the small protein ubiquitin (Ub) to membrane proteins by the membrane-associated RING-CH (MARCH) family of ligases is an important mechanism of control of membrane immunoreceptors. This project will employ biochemical techniques, microscopy, proteomics, and CRISPR-Cas9 technology to characterise the function of the MARCH family; identify novel MARCH substrates; and characterise the machinery involved in ubiquitination by MARCHs. The MARCHs have also been shown to play an important role in control of infection by HIV and other enveloped viruses. Our goal is to develop novel therapeutic approaches to fight infection based on manipulation of membrane protein ubiquitination. 

Contact project supervisor for further
information and application enquiries

Project Supervisor

Professor Jose Villadangos

Project Co-supervisor

Dr Justine Mintern

Project availability
PhD/MPhil
Master of Biomedical Science
Honours

Villadangos Group

[email protected]

3 vacancies

Themes
Host Pathogens Interactions
Immunology
Cross Cutting Disciplines
Translational and Clinical Research
Discovery Research

Research Projects 2019 | 57 The Villadangos group studies the first event that triggers adaptive immune responses: the presentation of pathogen or tumour antigens to T cells by dendritic cells, B cells and macrophages. We are characterising the development, regulation and impairment of antigen presenting cells by pathogens, inflammatory mediators and tumours. We are also dissecting the biochemical machinery involved in antigen capture, processing and presentation. We use this knowledge to understand how T cell-dependent immunity is initiated and maintained, and apply it to design better vaccines and immunotherapies against infectious agents and cancer. 


Villadangos Group Current Projects